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1.
Mongolian Pharmacy and Pharmacology ; : 11-15, 2015.
Article in English | WPRIM | ID: wpr-975989

ABSTRACT

Introduction:Saxifraga spinulosa Adams (S.spinulosa) belongs to the Saxifragaceae family and it is used for treating conjunctivitis, gynecopathy, larynx, pleural cavity in traditional Tibetan and Russian medicine. Study on antioxidant activity on this plant have also been carried out previously. However, no work has been reported on its anti-acetylcholinesterasic and anti-quorum sensing activity.Goal: The purpose of this research was to perform phytochemical, anti-acetylcholinesterase and anti-quorum sensing activities on the extracts of aerial parts of Saxifraga spinulosa Adams.Methods and materials: Anti-acetylcholinesterase activity was evaluated by colorimetric method. Extracts of aerial parts of S.spinulosa were tested for anti-quorum sensing activity by the Chromobacterium violaceum assay using the standard disc-diffusion method. Extracts were chromatographed with thin layer chromatography method. Phytochemical screening of crude extracts S. spinulosa Adams was carried out to detect saponins (Kokate, 1999), ferric chloride and lead acetate test was for tannin and phenolic contents and the detection of gum (Whistler, 1993) and Fehling test of carbohydrates respectively. Results: Plant extraction and fractionation was made earlier. Acetylcholinesterase activity screening was performed on its 4 extracts, prepared from aerial parts of Saxifraga spinulosa Adams. Non-polar chloroform fraction showed more active with 45.7% compared to the aceton extract (36%), water residue (35,5%) and n-butanol fraction (37,5%). Anti-quorum sensing activity was observed with 7 extracts of S. spinulosa aerial part. From them, fractions A (21 mm), B (20 mm), C (20 mm) showed significant activity and others were moderate. No antibacterial activity (for Chromobacterium violaceum) was observed. Thin layer chromatography (TLC) fingerprinting indicated the presence of the phenolic components and flavonoids. Phytochemical screening revealed that phenolic contents, tannins, saponins, carbohydrates, gums were present in the whole plant. Conclusion: This study shows that, S. spinulosa has anti-acetylcholinesterase and anti-quorum sensing activity and we determined that this plant mainly contains polyphenolics compounds. Key words: anti-quorum sensing, anti-acethylcholinesterase, Saxifraga spinulosa Adams

2.
Mongolian Pharmacy and Pharmacology ; : 11-15, 2014.
Article in English | WPRIM | ID: wpr-975969

ABSTRACT

Introduction: Oxidative stress occurs when the production of harmful molecules called free radicals are beyond the protective capability of the antioxidant defenses. Free radicals are known to cause damage to lipids, proteins, enzymes and nucleic acids, they are leading to cell or tissue injury. Therefore, there is an increasing interest in searching antioxidants from natural origin to prevent human body from oxidative stress produced by free radicals. In this research, 52 extractions of Mongolian medicinal plants were tested for their DPPH radical scavenging activity. Out of them, the aerial parts of Saxifraga spinulosa showed significant activity. Phytochemical and biological studies on this plant haven’t been determined previously. Methods: Antioxidant activity was evaluated by DPPH (2,2- diphenyl-1-picrylhydrazyl) radical scavenging assay. The antioxidant activity of these extracts was compared with standard solution of rutin. The total phenolic contents, flavonoid, and flavonols were determined by Folin-Ciocalteu, aluminum chloride, and Yermakov methods, respectively. Results: The acetone extract of the aerial parts of Saxifraga spinulosa exhibited excellent scavenging activity with IC50 (μg/mL) 14.98±0.16 against DPPH radical. Moreover the n-butanol fraction showed greater DPPH scavenging activity with IC50 (μg/mL) 28.69±0.09 compared to the non-polar chloroform fraction. So far the active n-butanol fraction passed through an octadecylsilicagel (3 x 12.5 см) and eluted with H20 (fraction A, 1.9 g), MeOH 20% (fraction B, 0.45 g), MeOH 40% (fraction C, 1.56 g), MeOH 80% (fraction D, 0.268 g), and MeOH (fraction E, 0.0012 g). From them, fractions A, B, C, and D showed significant activity, while fraction E had moderate activity. The total phenolic contents of acetone extract (121.42±14.33), n-butanol (113.42±16.29), and water residue (68.09±20.44), were expressed as Gallic acid equivalents. The flavonoid contents were found to be acetone extract (37.27±3.05), n-butanol (50.6±6.43), and water residue (5.93±2.0) in methanol using rutin equivalents. Conclusions: Antioxidant activity of Saxifraga spinulosa extracts were shown to be related to the phenolic components. Detailed investigation is needed to isolate antioxidant active compounds from this plant and determine their chemical structures as well as to make it accessible to modern clinical practice. Key words: Saxifraga spinulosa, DPPH radical scavenging activity, total phenolic content

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